Anti-DYKDDDDK-tag agarose is high-affinity mouse IgG1 monoclonal Anti- DYKDDDDK-tag antibody covalently coupled to the surface of agarose. It is useful for purification or immunoprecipitation of DYKDDDDK-tag fusion proteins.
||High-affinity mouse IgG1 monoclonal Anti- DYKDDDDK-tag antibody
||4% cross-linked agarose beads
||The particle size range is 45-165 μm, and the average particle size is 90 μm
||>1mg/mL flag resin (70kDa fusion protein)
||This antibody recognizes DYKDDDDK-tag at N-terminal, Met-N-terminal, C-terminal, and internal locations of a DYKDDDDK-tag fusion protein
||Suspension medium, bead is white, solution is colorless
||0.5-1.0 mL/min is recommended for 1mL colum
||≤ 0.3 mpa
1. Higher binding capacity then competitor;
2. More stable and higher binding capacity then competitor;
3. Suitable for most of the DYKDDDDK tagged protein;
4. Suitable for both N-terminal and C-terminal tagged protein;
5. Suitable for low or highly viscous samples
6. Suitable from small to bulk of sample's purification
7. Can be used in a column chromatography, batch Chromatography and immunoprecipitation
8. Spot stocks with strict quality control
Anti-DYKDDDDK-tag agarose is shipped at ambient temperature. Please store it at 2~8℃or –20℃
This agarose is stable for one year from the date of purchase when stored at -20 ℃. After use, the resin should be cleaned and stored in 50% glycerol with PBS buffer containing 0.02% sodium azide to protect the product. Do not freeze in the absence of glycerol. Avoid repeated freezing and thawing.
Anti-DYKDDDDK-tag agarose is supplied as a50% slurry in PBS, pH 7.4, containing 50% glycerol and 0.02% (w/v) sodium azide.
Reagents Required but not Provided:
Binding Buffer: PBS (10 mM phosphate, 2.7 mM potassium chloride, and 137 mM sodium chloride, pH 7.4)
Elution Buffer: 100 mM Glycine, 10 mM NaCl, pH 3.0
Neutralization Buffer: 1M Tris-HCl, pH8-9
Fig.1. Binding capacity comparison between competitor(A) and Sinobiological's resin(B)：The competitor(A) and Sinobiological's resin(B) were incubated with the same amount of DYKDDDDK tagged protein(>2 mg, excessive)and purificated according to the instruction respectively. The binding capacity was checked by 13% SDS-PAGE and UV-Vis.
Fig.2.Suitable for both N-terminal and C-terminal tagged protein:Sinobiological's resin was used in the purification of sample with protein A\B\C\D\E (C-terminal tagged) and protein F (N-terminal tagged). The purification result was checked by 13% SDS-PAGE and UV-Vis.
Fig.3.Suitable for repeated usage of low viscous samples' purification:1mL of the competitor(A) and Sinobiological's resin(B) was used in the purification of the same amount of low viscous sample(20mL) to get the target protein(about 80kD heterologous dimmer, one of the molecule is about 40kD with a C-terminal DYKDDDDK tagged) for 20 times respectively. The binding capacity was checked by 13% SDS-PAGE and UV-Vis.
Fig.4.Suitable for repeated usage of high viscous samples' purification:1mL of the Sinobiological's resin was used in the purification of the high viscous sample(20mL) to get the target protein(about 40kD, C-terminal DYKDDDDK tagged) for 6 times. The binding capacity was checked by 13% SDS-PAGE and UV-Vis.