Baculovirus-Insect Cell Protein Expression Service

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Baculovirus-Insect Cell Protein Expression Service Introduction

Baculovirus-insect cell system belongs to eukaryotic expression system, with capability of protein folding and modification after protein translation. It is suitable for expressing proteins that are probably harmful to mammalian host cells, such as kinases and toxic proteins. Sino Biological possesses abundant experience of baculovirus-Insect cell protein expression, especially the expression of large proteins, toxic proteins, kinases, intracellular proteins and membrane proteins.

Baculovirus-Insect Cell Protein Expression Service Advantages

Over 10 years' experience of protein expression
One-stop service from gene sequence to purified protein
High-efficiency expression of intracellular and extracellular proteins
Multiple insect cells: Sf9,Sf21,Hi-5
High-throughput and large-scale protein production
Optimized expression vectors and higher-titre virus packaging

Baculovirus-Insect Cell Protein Expression Service Procedures

Communicate about protein expression requirements  Sign contract and make prepayment  Carry out pilot study for protein expression  Feedback pilot study result and ship sample (if feasible)  Bulk production quotation  Bulk production based on customer requirements

Baculovirus-Insect Cell Protein Expression Service Items

Service procedures and items Cycle Delivery
Vector construction 1-2 weeks Sequencing report
• clone cDNA into expression vector
• plasmid sequencing
• large-scale plasmid preparation
Virus packaging and amplification 2-3 weeks  
• virus packaging
• virus amplification
Expression and purification 2-3 weeks • Feasibility report
• 0.1-0.5 mg sample (If expression is feasible and sample remains from QC testing)
• virus infecting cells
• small expression, purification and condition optimization
• SDS-PAGE and UV analysis
Large-scale expression and purification (optional) 2-3 weeks • Purified protein
• SDS-PAGE report
• CoA