Antibody Production & Development Service

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CRO Service
Recombinant Protein Production Service
E.coli expression service
Mammalian Transient Protein Expression Service
Yeast expression service
Baculovirus-Insect Cell Protein Expression Service
HEK293/CHO Stable Cell Line Development Service
Antibody Development & Production Service
High-throughput Antibody Production Service+
Mini-scale Antibody Production Service(0.1mg-1mg)
Small-Mid scale Recombinant Antibody Production Service(1mg-100mg)
Large-scale Recombinant Antibody Production Service
Rabbit Monoclonal Antibody Development Service
Mouse Monoclonal Antibody Development Service
Rabbit Polyclonal Antibody Development Service
Hybridoma Cell Culture And Antibody Production Service
Hybridoma Antibody Gene Sequencing Service
Antigen Production Service
ELISA Kit Development Service
Biopharmaceutical Service
Therapeutic Antibody Development Service+
Protein antigen production
Peptide design & synthesis
Mouse monoclonal antibody development
Rabbit monoclonal antibody development
Hybridoma cell culture and antibody production
Monoclonal antibody sequencing
High-throughput antibody production
Antibody Engineering Service+
Chimeric antibody production service
Antibody fragment production service
Bispecific antibody production service
Anti-idiotype antibody development service
Biopharmaceutical Analysis Service
Bioprocess Engineering Service

Custom Antibody Production & Development Service Introduction

Sino Biological has more than 10 years experience in antibody development and production, and is committed to providing scientific research institutions and pharmaceutical companies with one stop services including antigen production, antibody development, ELISA kit development, antibody detection and production, hybridoma antibody gene sequencing, chimeric antibody and antibody fragment expression, antibody humanization, antibody affinity maturation, anti-idiotype antibody and bispecific antibody production services.

Most Popular Antibody Production Service

Mini-scale
Antibody Production Service
(0.1mg-1mg)

Timeline:2-3 weeks

  • • Free codon optimization
  • • Optimized high-efficiency expression vectors
  • • Optimized culture medium and expression systems
  • • Flexible production scales
  • • Fast turnaround
  • • Great expression capacity: >100 antibodies per week
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Small and Mid-scale
Antibody Production Service
(1mg-100mg)

Large-scale
Antibody Production Service
(100mg-100g)

Timeline:3-6 weeks

  • • Self-developed high-efficiency culture medium and transfection reagents, high-expression vectors and engineering cell lines with indigenous intellectual property, ensuring high success rates at low cost for antibody production
  • • Different culture scale from shake flasks to bioreactors, 24 hours a day, 7 days a week continuous culturing to ensure rapid production of antibodies
  • • Provide transient transfection for antibody production, and develops stable cell lines for antibodies production to meet different customers needs
  • • Express full-length antibody of each subtype (IgG1, IgG2a, IgG2b, IgG3, IgG4, IgM, etc.), chimeric antibody (human/mouse chimeric antibody, mouse/rabbit chimeric antibody, etc.), and also antibody fragments (Fab, scFv, nanobody, etc.)
  • • Produce antibodies with high-purity, high-concentration and low-endotoxin according to customers demands
  • • One-stop Service: Mab development- Hybridoma antibody gene sequencing - Mouse antibody production - Chimeric antibody production - Antibody humanization - Antibody affinity maturation - Antibody characterization and analysis- Process development - GMP production

Antibody Production & Development Service Items

Antibody Production & Development Service

Advantage

  • Recombinant antibody production is a key technology for the industrialization of monoclonal antibody drugs. Without immunization and screening process, antibody gene sequence can be directly & stably used to produce large amounts of antibodies. Sino Biological has a complete recombinant antibody production platform and much experience on the production of recombinant antibodies. We can provide rapid production of recombinant antibodies ranging from milligrams to kilograms, meeting the high throughput and large-scale production requirements of customers.
• Mini-scale Antibody Production Service        • Small & Mid-scale Antibody Production Service        • Large-scale Antibody Production Service

Rabbit Mab development Service

Advantage

  • • International leading recombinant protein expression & purification technology, multiple protein expression & purification platforms, rich experience on 6000+ protein production, high success rate of antigen expression
  • • Professional peptide design software and high-efficiency conjugation methods ensure that the immune success rate of designed peptide is greater than 95%
  • • Leading antibody library construction and screening technology, reduce the risk of antibody fragment deletions or mismatches and increase the probability of screening for target antibodies
  • • ELISA, WB, IHC, FACS and other validation platforms, multi-cell and multi-tissue verification, aiming to improve antibody screening success rate with low background and high sensitivity
  • • High affinity, 10-100 times more than mouse mAb, in average

Mouse Mab development Service

Advantage

  • • Professional peptide design software and high-efficiency conjugation methods ensure that the immune success rate of designed peptide is greater than 95%
  • • Customized immunization, screening and detection proposed according to customer's final application requirements can be provided
  • • ELISA, WB, IHC, FACS and other validation platforms, multi-cell and multi-tissue verification, aiming to improve antibody screening success rate with low background and high sensitivity
  • • Quoting by steps, charging by nodes, reducing customer's risk and saving costs
  • • Optimized electrofusion technology has more than 3-5 times efficiency compared with PEG mediated cell fusion

Rabbit Pab development Service

Advantage

  • • International leading recombinant protein expression & purification technology, multiple protein expression & purification platforms, rich experience on 6000+ protein production, high success rate of antigen expression
  • • Professional peptide design software and high-efficiency conjugation methods ensure that the immune success rate of designed peptide is greater than 95%
  • • Serum titer ≥1:64,000
  • • Quoting by steps, charging by nodes, reducing customer's risk and saving costs

Hybridoma cell culture & antibody production Service

Advantage

  • • > 200 hybridoma cultures and antibody production experience, success rate >99%。
  • • The scale of production ranges from milligrams to grams, meet the demands of different customers experiments
  • • Rich experience on the production of various antibody subtypes including IgG1, IgG2a, IgG2b, IgM, etc.
  • • The high quality culture medium produced in house can meet the need of high density culture, increase yield and reduce cost
  • • Optimize culture and purification process to ensure the rapid and efficient purification of antibodies

Hybridoma antibody gene sequencing Service

Advantage

  • • Cloning of heavy- and light- chain variable region gene of mAb or antibody full-length gene
  • • Rich experience on primer design and antibody gene analysis
  • • Multiple species (human, mouse, rat, rabbit, etc.) and subtypes (IgG1, IgG2a, IgG2b, IgM, etc.) can be used for antibody gene sequencing
  • • One-stop service from antigen production - antibody development - hybridoma antibody gene sequencing - chimeric antibody production to humanized antibody development etc.

Antigen produciton Service

Advantage

  • • International leading recombinant protein production and purification technology, with five antigen expression platforms: HEK293, CHO, E.coli, Yeast, and Insect cell, and multiple protein purification
  • • With intellectual property of medium, highly efficient expression vector and high-density cell culture systems to fully improve expression success rates and save customers cost
  • • The leading technical personnel and laboratory equipment, rich experience on 6000+ protein expression and purification, supporting high-throughput, large-scale and rapid production
  • • Professional polypeptide design software, experienced designers, and efficient conjugation solutions to ensure the immunization successful rate of the designed polypeptide is greater than 95%

ELISA Kit development Service

Advantage

  • Abundant resources:Sino Biological has developed nearly 30,000 antibodies covering 2,200 targets and has a protein library with >6000 proteins; We have 5 major protein expression and production platforms including HEK293, CHO, E.coli, Yeast, and Insect cell, As well as optimized reagent formulation, enzyme labeling technology and production process. Sino Biological can also customize related products and carry out kit development according to customers' needs in least time.
  • Professional R&D platform:Sino Biological has developed more than 100,000 ELISA antibody in the past 10 years. We have successfully developed 400+ ELISA pair set and 150+ ELISA kits. Sino Biological also has rich experience on ELISA kit customization for dozens of biopharmaceutical companies.
  • Professional technical support:Our techincal support team will provide suitable proposal to meet customers' need fastly and efficiently.
  • Quality assurance:We have various natural samples, as well as testing experience which can be used for comprehensive and accurate methodological validation of Elisa kits.
  • Convenient and fast:We can provide one-stop service with less time lines and high efficiency, which can help customer save time

Custom Antibody Production & Development Service Advantages

Varied expression platforms for antigen:Five antigen expression platforms including HEK293, CHO, E.coli, Yeast,and Insect cell can meet the needs of different types of antigen recombinant expression
Multiple antigen types:Polypeptide and protein antigens are two main methods for immunization and screening, various methods such as DNA, cell and insect virus immunization are also available
Various animal species for immnization:Mouse and rabbit
Different antibody development technologies:Hybridoma technology and antibody library screening technology can be used to increase the probability of obtaining positive clones and high-specificity & high-affinity antibodies
Diversified antibody screening technologies:Solid-phase and liquid-phase antibody screening techniques can both be used to reduce the amount of coating antigen and increase antibody diversity
Multiple antibody detection platforms: ELISA, WB, IHC, FACS, SEC-HPLC, Octet, endotoxin, mass spectrometry and other antibody detection platforms can be used for comprehensive assessment of antibody quality
Various antibody purification methods:Protein A, protein G, protein L, size exclusion chromatography, ion exchange chromatography, antigen affinity purification and other purification methods will be done to improve antibody purity
Antibody recombinant expression technology:Using optimized plasmids and HEK293 cell line, Sino Biological enables rapid, high-throughput and large-scale production of recombinant antibodies and antibody fragments
Hybridoma culture technology:Serum-free suspension culturing of hybridoma cells can be used, compared with ascites, the antibody will not include complex components and will avoid the serum influence on detection