Large-scale Recombinant Antibody Production Service

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Recombinant Antibody Production Service Introduction

Recombinant antibody production is a key technology for the industrialization of monoclonal antibody drugs. Without immunization and screening process, antibody gene sequence can be directly & stably used to produce large amounts of antibodies. Sino Biological has a complete recombinant antibody production platform and much experience on the production of recombinant antibodies. We can provide rapid production of recombinant antibodies ranging from milligrams to kilograms, meeting the high throughput and large-scale production requirements of customers.

Recombinant Antibody Production Service Advantages

Self-developed high-efficiency culture medium and transfection reagents, high-expression vectors and engineering cell lines with indigenous intellectual property, ensuring high success rates at low cost for antibody production
Different culture scale from shake flasks to bioreactors, 24 hours a day, 7 days a week continuous culturing to ensure rapid production of antibodies
Provide transient transfection for antibody production, and develops stable cell lines for antibodies production to meet different customers needs
Express full-length antibody of each subtype (IgG1, IgG2a, IgG2b, IgG3, IgG4, IgM, etc.), chimeric antibody (human/mouse chimeric antibody, mouse/rabbit chimeric antibody, etc.), and also antibody fragments (Fab, scFv, nanobody, etc.)
Produce antibodies with high-purity, high-concentration and low-endotoxin according to customers demands
One-stop Service: Mab development- Hybridoma antibody gene sequencing - Mouse antibody production - Chimeric antibody production - Antibody humanization - Antibody affinity maturation - Antibody characterization and analysis- Process development - GMP production

Recombinant Antibody Production Service Process

Description Timeline Deliverables
Gene synthesis and codon optimization (optional) 1-2 weeks • Purified antibody
• SDS-PAGE data
• CoA
Vector construction 1-2 weeks
• Cloning the HC and LC gene into optimized expression vectors, respectively
• Plasmid sequencing
• Large scale plasmid production
Expression & purification 1-2 weeks
• Transient transfection of HEK293/CHO cells
• Antibody purification
• Polishing if needed
QC analysis 3 days
• Analysis by SDS-PAGE and UV
• Purity analysis by HPLC-SEC and endotoxin detection
(optional)

In this large-scale recombinant antibody production service, customers need to provide:

Antibody gene sequence or vectors containing antibody gene