Mammalian Transient Protein Expression Service (HEK293 / CHO Cell)

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Mammalian Transient Protein Expression Service Features

Mammalian cells are capable of protein folding and post-translational modification, which can express proteins with molecular structures, physical and chemical structures, and biological functions mostly approaching natural higher-orgainism proteins. This bring more possibility to obtain the same bioactivity as natural protein. Sino Biological mammalian transient expression system, mainly using CHO cells and HEK293 cells as expression hosts, can provide flexible services which could meet different custom needs, including tag removal, endotoxin removal, SEC-HPLC assay、glycosylation analysis and so on.

Mammalian Transient Protein Expression Service Advantages

High-efficiency expression vectors
Over 10 years' experience for protein expression
Optimized mammalian expression systems
One-stop service from gene sequence to purified protein
High-throughput and large-scale production
High yield expression with post-translational modification and correct folding

Mammalian Transient Protein Expression Service Procedures

Communicate about protein expression requirements  Sign contract and make prepayment  Carry out pilot study for protein expression  Feedback pilot study result and ship sample (if feasible)  Bulk production quotation  Bulk production based on customer requirements

Mammalian Transient Protein Expression Service Items

Service procedures and items Cycle Delivery
Gene synthesis and codon optimization (optional) 1-2 weeks  
Vector construction 1-2 weeks Sequencing report
• clone cDNA into expression vector
• plasmid sequencing
• large-scale plasmid preparation
Expression and purification 2-3 weeks • Feasibility report
• 0.1-0.5 mg sample (If expression is feasible and sample remains from QC testing)
• transient transfection of HEK293/CHO cells
• protein purification
• SDS-PAGE and UV analysis
Large-scale expression and purification (optional) 2-3 weeks • Purified protein
• SDS-PAGE report
• CoA