Mouse Monoclonal Antibody Development Service

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Mouse mAb Development Service Introduction

Mouse mAb has many features such as targeting to a single epitope, high specificity, stable passage, large-scale repeated production and extensive applications in basic research, medical diagnosis, tumor therapy and radioimmunoimaging. Mouse mAb developed by Sino Biological has the advantages of high sensitivity & specificity. We can also provide flexible customized solutions according to customer requirements, aiming to provide customers with high-quality antibodies.

Mouse mAb Development Service Advantages

International leading recombinant protein expression & purification technology, multiple protein expression & purification platforms, rich experience on 6000+ protein production, high success rate of antigen expression
Professional peptide design software and high-efficiency conjugation methods ensure that the immune success rate of designed peptide is greater than 95%
Customized immunization, screening and detection proposed according to customer's final application requirements can be provided
ELISA, WB, IHC, FACS and other validation platforms, multi-cell and multi-tissue verification, aiming to improve antibody screening success rate with low background and high sensitivity
Quoting by steps, charging by nodes, reducing customer's risk and saving costs
Optimized electrofusion technology has more than 3-5 times efficiency compared with PEG mediated cell fusion

Mouse mAb Development Service Process

Description Timeline Deliverables Guarantee
Antigen preparation Please refer to protein expression service
Antigen validation 1-2 days • 2 positive clones
• 0.1-0.5mg purified mAb per clone
• 2 tubes of frozen hybridoma cells per clone
• CoA
• Remaining antigen
ELISA positive for immunogen
• Analysis of client's antigen by SDS-PAGE and UV
Immunization and serum titer test 8-10 weeks
• Pre-immune bleed
• Five Balb/c mice immunization
• Serum titer test
• Final bleed
Fusion and Screening 4-6 weeks
• Fusion and Screening
• Subcloning and cell expansion
• Cell Banking
Antibody production & purification 2-3 weeks
• Appropriate scale hybridoma cell culture
• Purified by protein A affinity chromatography
QC analysis 3 days
• Analysis by SDS-PAGE and UV
• ELISA validation

User-supplied antigen requirements:

Recombinant protein antigen
Antigen quantities 3-4mg/5 mice
Antigen Size >10kD
SDS-Page purity >90%
Concentration >0.5mg/mL
Formulation PBS, if not PBS, please inquire first
Peptide antigen
• KLH/VLP conjugated peptides 5mg/5 mice
Concentration >0.5mg/mL
• OVA/Biotin conjugated peptides 2-3mg
Concentration >0.1mg/mL
Formulation PBS, if not PBS, please inquire first