Rabbit Monoclonal Antibody Development Service

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Protein antigen production
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Rabbit mAb Development Service Introduction

Rabbit mAb usually has higher affinity and specificity than mouse mAb, it can recognize more novel epitopes and mouse antigens, and plays a key role in the development of therapeutic drug candidates and diagnostic kits. Sino Biological develops rabbit mAb by constructing antibody library to directly obtain antibody genes and their sequences, which is more suitable for the long-term preservation of excellent antibodies and their recombinant expression.

The Second Generation Rabbit mAb Technology

The Second Generation Rabbit mAb Technology
High affinity
High specificity and low background
Identify more novel epitopes
Larger antibody libraries
Recognize murine antibodies
Convenient to be humanized
The second-generation rabbit mAb can be directly modified for genetic engineering

Rabbit mAb Development Service Advantages

International leading recombinant protein expression & purification technology, multiple protein expression & purification platforms, rich experience on 6000+ protein production, high success rate of antigen expression
Professional peptide design software and high-efficiency conjugation methods ensure that the immune success rate of designed peptide is greater than 95%
Leading antibody library construction and screening technology, reduce the risk of antibody fragment deletions or mismatches and increase the probability of screening for target antibodies
ELISA, WB, IHC, FACS and other validation platforms, multi-cell and multi-tissue verification, aiming to improve antibody screening success rate with low background and high sensitivity
High affinity, 10-100 times more than mouse mAb, in average
One-stop service: From antibody development, vector construction to antibody expression & purification, saving customers time
Quoting by steps , charging by nodes, reducing customer's risk and saving costs

Rabbit mAb Development Service Process

Description Timeline Deliverables Guarantee
Antigen preparation Please refer to protein expression service
Antigen validation 1-2 days • Antibody H and L chains sequences for 1 positive clone
• 0.1-0.5mg purified mAb
• Vectors containing H and L chain, respectively
• CoA
• Remaining antigen
ELISA positive for immunogen
• Analysis of client's antigen by SDS-PAGE and UV
Immunization and serum titer test 8-10 weeks
• Pre-immune bleed
• 2 rabbits immunization
• Serum titer test
• Final bleed
Library construction & screening 4-6 weeks
Antibody production & purification 2-3 weeks
• Vector construction
• Transient transfection of HEK293 cells
• Purified by protein A affinity chromatography
QC analysis 3 days
• Analysis by SDS-PAGE and UV
• ELISA validation

User-supplied antigen requirements:

Recombinant protein antigen
Antigen quantities 2.5-4mg/rabbit
Antigen Size >10kD
SDS-Page purity >90%
Concentration >0.5mg/mL
Formulation PBS, if not PBS, please inquire first
Peptide antigen
• KLH/VLP conjugated peptides 3-5mg/rabbit
Concentration >0.5mg/mL
• OVA/Biotin conjugated peptides 2-3mg
Concentration >0.1mg/mL
Formulation PBS, if not PBS, please inquire first