Stable Cell Line Development Service (HEK293 / CHO)

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HEK293/CHO Stable Cell Line Development Service
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Stable Cell Line Development Service Introduction

Stable cell line is useful in various areas, such as recombinant protein and antibody production, testing and analysis, gene editing, functional study and so on. Engaged in protein expression study for years and possessing abundant stable cell line development experience, Sino Biological can effectively increase stable cell line productivity and improve experimental results. Integrating all the experimental steps, we provide one-stop service from gene sequence to delivered stable cell line.

Stable Cell Line Development Service Procedures

Experimental requirements and price communication  Contract signing and payment in advance  Transient expression evaluation (CHO cell)   Stable cell line construction and selection   Stable cell line providing  Large-scale protein expression and purification (optional)   Product delivery and payment in the rest

Stable Cell Line Development Service Items

CHO stable cell line development

Service procedures and items Cycle Delivery
Gene synthesis and codon optimization (optional) 1-2 weeks  
Vector construction 2-3 weeks Sequencing report
• cloning cDNA into expression vector
• plasmid sequencing
• large-scale plasmid preparation
Transient expression evaluation 2 weeks Evaluation report
• transient expression
• expression level detection by ELISA or WB
Stable cell line development 12-20 weeks • Expression report
• 2-3 stable clones (optional)
• stable transfection and screening
• MTX amplification and screening
• high expression clone screening and stabllity screening
• cell bank construction
Large-scale expression and purification (optional) 2-3 weeks • Purified protein
• SDS-PAGE report
• CoA

HEK293 stable cell line development

Service procedures and items Cycle Delivery
Gene synthesis and codon optimization (optional) 1-2 weeks  
Vector construction 2-3 weeks Sequencing report
• cloning cDNA into expression vector
• plasmid sequencing
• large-scale plasmid preparation
Stable cell line construction 10-12 weeks • Identification report
• 2 tube cells(1×10^6 cells / tube)
• stable transfection and screening
• Identification (qPCR and / or FACS)
• Subcloning
• Identification (qPCR and / or FACS)
• cell bank construction
Large-scale expression and purification (optional) 2-3 weeks • Purified protein
• SDS-PAGE report
• CoA